Aircraft fuel measurement system is an important part of aircraft fuel system,which is used to measure and indicate the available fuel amount of each tank and the total available fuel amount of aircraft. Fuel measurement accuracy is an important performance index of aircraft fuel measurement system,which can be divided into ground attitude and in the air. The accuracy of ground aircraft fuel measurement system can be calibrated by the method of ground calibration test. Since the actual fuel quantity of aircraft cannot be known in the air,it is impossible to calibrate the accuracy of aircraft fuel measurement system in the air through test method,the accuracy of aircraft fuel measurement in the air should be explained by combing the accuracy of ground calibration and air analysis. This paper introduces the calibration method of fuel measurement system of large civil aircraft in ground attitude,including the selection of standard point,calibration method,qualification criterion,data processing and so on.