ARP4754 is the most important development assurance standard for the development of civil aircraft and systems, and the revision of the requirements of development assurance has received extensive attention from civil aircraft development units and airworthiness authorities of various countries. In view of the latest ARP4754B issued by SAE, a detailed comparison and analysis of ARP4754B with ARP4754A is carried out. The background and content of the revision of ARP4754B are comprehensively studied. This paper gives key points of revision in combination with the discussion of the revision of ARP4754B, and gives follow-up development assurance implementation suggestions for development assurance planning, aircraft and systems development process, integral process, and change implementation in combination with the aircraft development experience and airworthiness review, so as to provide an effective reference for the development unit to better understand the requirements of ARP4754B development assurance, and to truly implement the ARP4754B in product development.