To study the effect of active noise control technology in the real flight environment of turbofan aircraft, an active noise control system based on adaptive filtering algorithm was designed, and integrated on a test aircraft. The test flight has the same cabin with the route aircraft. We select the flight test in typical flight condition of route aircraft, including climbing and cruising in different height. The test data shows that the active noise control system still has obvious noise reduction effect in the complex cabin and flight environment. And the noise reduction effect is more related to the basic noise, usually to adjust the spatial noise to coincide. The greater the basic noise is, the better the noise reduction effect is. The noise control effect of low frequency is stabler and better than high frequency. Also we give advices on the layout of the active noise control system on the turbofan aircraft.