For the cargo compartment smoke detection system in Combi aircraft, its function verification test has not yet been carried out in China, and there is no corresponding test standards. In order to evaluate the smoke detection system in a Combi aircraft cargo compartment, the research and development tests were carried out using the smoke detection test method commonly used by civil transport aircraft for reference, and the results showed that the test method was not applicable. In order to reformulate the test method, the characteristics of different types of smoke generators were analyzed, and suggestions for the selection of smoke generators were given. According to the characteristics of the smoke detection system in the cargo cabin of the aircraft, a new method to determine the amount of smoke and to select the smoke location was proposed. Based on the operating environment of the cargo, the test conditions were determined, the final test method was formed, and the assessment of the cargo compartment smoke detection system was completed. This test method can provide reference for the cargo compartment smoke detection system test in Combi aircraft.