Aiming at the problem that there is no corresponding reference standard for the alpine airworthiness test in the domestic civil aircraft laboratory, it was studied that verification technology of the alpine airworthiness test in the civil aircraft laboratory in this paper. Firstly, the airworthiness clauses 25.1301 (a) (4), 25.1309 (a) and M-6 (a) related to the cold climate environment of civil aircraft were briefly interpreted, and the airworthiness requirements for the cold test verification of civil aircraft were given. By analyzing the implementation process of a civil aircraft laboratory cold test, the laboratory test verification process suitable for the airworthiness compliance of civil aircraft was put forward. Secondly, several key problems existing in the implementation of civil aircraft complete machine laboratory Alpine test were studied, mainly including aircraft installation and mooring mode, test items, test sequence and test condition determination method, test scheme framework, test procedure optimization method, etc. Finally, combined with the test method, the judgment principle of the test results was given, and a verification method suitable for the cold airworthiness test of civil aircraft in the laboratory was formed. This method could provide technical support for the effective implementation of laboratory Alpine test of civil aircraft.