Airspeed calibration is directly related to the safety of civil aircraft first flight. Papers on first flight airspeed calibration were rare. This paper summarizes the significance of first flight airspeed calibration, systematically introduces the common first flight airspeed calibration methods such as trailing cone method, Pace-chase method and 4-leg GPS method and gives some suggestions on first flight preparation, first flight mission design, first flight safety guarantee and first aircraft group training based on my practical work experience. In addition, according to the actual characteristics of the first flight of domestic civil aircraft, recommendation on airspeed calibration during first flight preparation, test plan, test safety and crew training was introduced. A new airspeed calibration test method focused on civil aircraft first flight was presented. The results show that this method does not need special flight test action and special test modification. It can save valuable first flight time and greatly reduce the difficulty of implementation. It can be used as a reference for the first flight of other civil aircraft models.