Atmospheric disturbance is an important factor that affects aviation safety, which is mostly involved in airworthiness regulations. The airworthiness compliance assessment of atmospheric disturbance for civil aircraft has become an important part of civil aircraft airworthiness certification. Based on CCAR-25-R4,the airworthiness clauses of atmospheric disturbances were screened and summarized, the requirements of the airworthiness clauses of atmospheric disturbances related to handling stability characteristics were deeply analyzed and refined. Then the clause requirements were refined and quantified according to the military flight quality specification MIL-STD-1797B, and the control quality rating method (HQRM) was used dealing with the influence of atmospheric disturbance,which means the impact of atmospheric disturbance was considered in the form of correlation between the severity and the probability of occurrence. The applicability and effectiveness of the quantitative index and the HQRM method for the compliance assessment of the relevant airworthiness clauses were verified through calculation examples, and a method was proposed that defining the atmospheric disturbance as a factor that degrades the flight quality, combined with the quantitative index to make the corresponding flight quality requirements. Research result provides reference for the airworthiness compliance analysis and verification of atmospheric disturbance and the airworthiness certification work of relevant provisions during the aircraft design phase.