For the turbofan engine, the nacelle core cowl is the designated fire zone. If the accessory gear box(AGB) is mounted in the fan compartment, the fan compartment is the designated fire zone. As the wrap up structure of the fire zone, the fire zone and non-fire zone should be isolated to prevent the fire or high-temperature gas from getting into other areas to result in damage. The fireproof seal is the important part of the nacelle fire barrier, which locate in the nacelle component and between pylon and nacelle. The seals locate in the fire barrier, which should meet the fireproof requirement. The fire test is the commonly used airworthiness verification method at present. Starting from the requirements of fire protection and airworthiness, this paper analyzes the requirement of the fire test, including the test article, the test equipment and the test environment, and expounds the fire protection test method of seals in detail. The article provides references for the fire test of the nacelle fire barrier.